个人简介

徐建国，教授，“南湖学者”特聘教授，工学博士，博士后，硕士生导师，2017年6月博士毕业于福州大学化学学院，2017.6-2023.8人才引进在合肥工业大学食品与生物工程学院从事教学、科研工作，历任讲师（2017.6）、副教授（2019.12）。2023年9月以高层次人才引进到888集团官网工作，入选嘉兴市“星耀南湖”青年领军人才计划。主要从事功能核酸增敏感知体系构建，致力于疾病标志物分析、食品安全危害因子及环境危害物检测等方面的基础及应用研究，主持包括国家自然科学基金、浙江省自然科学基金重点项目、安徽省重点研发计划/面上/青年基金、中国博士后面上基金等科研项目10余项。已在本学科权威期刊Anal. Chem.、Adv. Sci.、Biomaterials.、Biosens. Bioelectron.、J. Hazard. Mater.、J. Agric. Food Chem.、Food Chem.、Crit. Rev. Food Sci.、Chem. Commun.等发表第一作者和通讯作者SCI收录论文60余篇(https://orcid.org/0000-0002-0187-2623)，获中国发明协会创业奖创新奖二等奖，参与制定地方标准3项/企业标准2项，授权发明专利2项（转让1项），参编中英文专著2部。兼任中科院一区Top期刊《Journal of Analysis and Testing》青年编委并获得2024年杰出青年编委奖，兼任中文核心期刊《分析试验室》编辑/青年编委，为Acs Nano、JACS Au、Nano Today、Small、Anal. Chem.、ACS Sens.、Biosens. Bioelectron.、Chem. Commun.、J. Agri. Food. Chem.、Food Chem.等领域内权威期刊审稿人。

Email: jgxu0816@163.com;

工作经历

研究方向

(1)     功能核酸探针设计与分子增敏感知平台构建

(2)     疾病标志物/食品安全危害因子/环境危害物分析检测

(3)     病原体检验检疫/抗菌技术构建、食品真实性鉴定

主持科研项目

(1)     浙江省自然科学基金重点项目，时间分辨等温扩增序贯激活CRISPR/Cas12a解码单色荧光分析多重食源致病菌，Z25C200003，2025.1.1-2027.12.31，主持

(2)     国家自然科学基金青年基金项目，基于功能集成式核酸探针的恶性肿瘤相关microRNAs多重信号放大诊断研究，21804028，2019.01-2021.12，主持

(3)     安徽省重点研发计划项目，现代农业中农产品农药残留及生物毒素质量控制快速检测标准制定研究，202004h07020029，2020.01-2022.12，主持

(4)     安徽省自然科学基金面上项目，DNA多维自组装耦合级联等温扩增的Salmonella荧光偏振增强传感平台构建，2308085MB57，2023.9-2026.8，主持

(5)     安徽省重点研发计划项目，安徽主产区稻米中典型重金属污染物的迁移规律与风险控制研究及应用，202104a07020008，2021.1-2023.12，子课题主持

(6)     安徽省重点研发计划项目，食管癌潜在生物标志物检测技术开发及其在术后复发监测方面的前瞻性研究，2022e07020060，2022.1-2024.12，子课题主持

(7)     安徽省自然科学基金青年项目，多功能核酸适配体探针多级信号扩增及痕量食源致病菌检测研究，1908085QC121，2019.07-2021.06，主持

(8)     中国博士后基金面上项目，改性分子信标及工具酶联用光电增敏检测循环microRNA，2019M652167，2019.06-2021.01，主持

(9)     嘉兴市社会发展专项，AI驱动的早期肠癌筛诊疗一体化多组学分子伴随诊断系统研发及应用，2025BS009，2025.04-2028.04，主持

代表性论文（时间倒序）

(1)     Zhuqi Sui, Baoqiang Chen, Jia Zhao, Haidong Yang, Longhua Guo, and Jianguo Xu*. Dual-Accelerated Signal Amplification in Biosensing via Spatial Confining Catalytic Hairpin Assembly-Activated Spherical CRISPR/Cas12a System for Trans-Cleavage of Hairpin DNA Reporters. Anal. Chem., 2025, 97, 8, 4668-4677

(2)     Hong Huang, Huiru Li, Yong Zhang, Xuhan Xia, Ningwen Zhang, Haixin Fan, Longhua Guo, Yongyong Cao, Hu Pan, Ruijie Deng*, Yangang Wang*, Rodrigo Ledesma-Amaro, Jianguo Xu*. Simultaneous Monitoring of Tyrosinase and ATP in Thick Brain Tissues Using a Single Two-Photon Fluorescent Probe. Adv. Sci., 2025, 2413220.

(3)     Pengpeng Xue, Yubo Peng, Renjing Wang, Qian Wu, Qi Chen, Chao Yan, Wei Chen*, and Jianguo Xu*, Advances, challenges, and opportunities for food safety analysis in the isothermal nucleic acid amplification/CRISPR-Cas12a era, Crit. Rev. Food Sci., 2025, 65(13), 2473-2488.

(4)     Qi Wang, Juan Xia, Chengying Yang, Xiumei Chen, Baoqiang Chen, Yang Li, Hong Huang, Bingyong Lin, Longhua Guo, and Jianguo Xu*. Cross-Priming-Linked Hierarchical Isothermal Amplification Programming Progressive Activating Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a in miRNA Signaling. Anal. Chem., 2024, 96, 35, 14205-14214.

(5)     Guohui Xue, Hong Huang, Lin Hua, Baoqiang Chen, Haiyan Dong*, Haiyan Jia, Chang Xue, and Jianguo Xu*. Tuning assembled DNA nanoarchitecture into disassembled state to enhance accurate and ultrasensitive detection of KRAS G12D mutation. Sensors Actuat. B-Chem., 2024, 420, 136513.

(6)     Jianguo Xu*, Tong Zhou, Danni Xue, Zhuqi Sui, Haidong Yang, Xinyue Yuan and Qi Wang*, Integrating recombinase polymerase amplification with CRISPR/Cas9-initiated nicking-rolling circle amplification in Staphylococcus aureus assay, Chem. Commun., 2024, 60, 5314 - 5317.

(7)     Qi Wang, Tong Zhou, Danni Xue, Haidong Yang, Zhuqi Sui, Xinyue Yuan, and Jianguo Xu*, An allosteric palindromic hairpin probe based dual-mode interactive strand displacement amplification enables robust miRNA biosensing, Chem. Commun., 2024, 60, 2910-2913.

(8)     Jianguo Xu*, Haidong Yang, Zhuqi Sui, Xinyue Yuan, Lee Jia, and Longhua Guo*, One-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity, Chem. Commun., 2024, 60, 4683-4686.

(9)     Qi Wang, Baoqiang Chen, Dan Zheng, Juan Xia, Lijun Wu*, and Jianguo Xu*. Intramolecular Accelerated Assembly of Molecular Beacons: A DNA Nanoarchitecture-based Spatial Confinement Strategy toward Terminal Deoxynucleotidyl Transferase Biosensing. Anal. Chem., 2023, 95, 13708-13715.

(10) Xinlei Zhang, Yubo Peng, Li Yao, Huijie Shang, Zhi Zheng, Wei Chen*, and Jianguo Xu*. Self-assembly of a multivalent aptamer-tethered DNA monolayers dedicated to a fluorescence polarization-responsive circular isothermal strand displacement amplification for Salmonella assay. Anal. Chem., 2023, 95, 2570-2578

(11) Huo Xu, Yanhui Zheng, Danlong Chen, Yinghao Cheng, Xiaojun Fang, Chunlian Zhong, Xinmei Huang, Qi Huang, Jiawei Xu, Jianguo Xu*, and Chang Xue*. A Branch-Shaped Trapping Device Regulates Accelerated Catalyzed Hairpin Assembly and Its Application for MicroRNA in Situ Imaging. Anal. Chem., 2023, 95, 1210-1218.

(12) Xiaoming Ma, Hui Zhang, Jin Liu, Huifang Zhang, Xuan Hu, Yu Wang, Xun Li, Jianguo Xu*. An ultrahigh-resolution multicolor sensing platform via target-induced etching of gold nanorods for multi-colorimetric analysis of trace silver ions. Sensors Actuat. B-Chem., 2023, 397, 134658.

(13) Huijie Shang, Yubo Peng, Li Yao, Zhi Zheng, Hongxia Li, Wei Chen*, Jianguo Xu*. Self-customized multi-channel exponential amplifications regulate powered monitoring of terminal deoxynucleotidyl transferase activity.Anal. Chem., 2022, 94, 32, 11401-11408

(14) Jianguo Xu*, Xinlei Zhang, Chao Yan, Panzhu Qin, Li Yao, Qi Wang, Wei Chen*. Trigging isothermal circular amplification-based tuning of rigorous fluorescence quenching into complete restoration on a multivalent aptamer probe enables ultrasensitive detection of Salmonella. Anal. Chem., 2022, 94, 2, 1357-1364

(15) Jie Wang Jie, Jiayin Sun, Jing Zhang, Chenlin Shen*, Xinlei Zhang, Jianguo Xu*. Engineered G-quadruplex-embedded self-quenching probes regulate single probe-based multiplex isothermal amplification to light road lamp probes for sensitized determination of microRNAs. Anal. Chem., 2022, 94, 10, 4437-4445

(16) Juan Xia, Zhaoqiang Liu, Shulin Gao, Wenyong Li, Qi Wang*, Jianguo Xu*, Hai Wu*. Intermolecular and intramolecular priming co-directed synergistic multi-strand displacement amplification empowers ultrasensitive determination of microRNAs. Anal. Chem., 2022, 94, 16132-16141

(17) Qi Wang #, Wenyong Li #, Juan Xia, Hai Wu, Lijun Wu*, Jianguo Xu*. Stepwise tuning of a molecular beacon coupled Y probe regulates ternary DNA nanomachine-based microRNA determination.Sensors Actuat. B-Chem., 2022, 363, 131858

(18) Xinlei Zhang, Jianguo Xu*, Xiuguang Xing, Li Yao, Huijie Shang, Wei Chen*. Framework nucleic acid-wrapped protein-inorganic hybrid nanoflowers with three-stage amplified fluorescence polarization for terminal deoxynucleotidyl transferase activity biosensing. Biosens. Bioelectron., 2021, 193: 113564

(19) Xiaoming Ma, Zhen Wang, Xuan Hu, Jinghua Chen, Huifang Zhang, Xun Li, Fengyan Xie, Jianguo Xu*. Nanozyme catalysis-powered portable mini-drainage device enables realtime and universal weighing analysis of silver ions and silver nanoparticles. J. Hazard, Mater., 2021, 415, 125689.

(20) Jianguo Xu*, Zhi Li, Yumei Li, Yusheng Lu, and Jie Wang*. Activation of palindromes on a degradable modular grafting probe enables ultrasensitive detection of microRNAs. Chem. Commun., 2021, 57, 5941-5944

(21) Xinlei Zhang, Jianguo Xu*, Chao Yan, Li Yao, Huijie Shang, Wei Chen*. Short- and long-range FRET co-functionalized fluorescence quenching collapsar probe regulates amplified and accelerated detection of Salmonella. J. Agric. Food Chem., 2021, 69, 14294-14301

(22) Xiaoming Ma, Shan He, Huifang Zhang, Xun Li*, Jun Xue, Xiaolin Fan, Yusheng Lu, Yiting Chen, Yanjie Zhang, and Jianguo Xu*. Delayed full opening of bumped switchable molecular probe enables repeated generation of target analogues for mix-to-signaling determination of microRNAs. Sensors Actuat. B-Chem., 2021, 327: 128875-128881

(23) Jie Wang, Shuhui Li, Jianguo Xu*, Yusheng Lu, Min Lin, Chaihung Wang, Chen Zhang, Guoxing Lin, Lee Jia*. Functionalized dumbbell probe-based cascading exponential amplification DNA machine enables amplified probing of microRNAs.Chem. Commun., 2020, 56, 1681-1684

(24) Wei Chen, Jun Teng, Li Yao, Jianguo Xu*, and Guodong Liu. Selection of specific DNA aptamers for hetero-sandwich based colorimetric determination of Campylobacter jejuni in food. J. Agric. Food Chem., 2020, 68, 31, 8455-8461

(25) Wei Chen*, Lijun Yang, Chao Yan, Bangben Yao, Jian-feng Lu, Jianguo Xu*, and Guodong Liu*. Surface-confined building of Au@Pt-centered and multi-G-quadruplex/hemin wires-surrounded electroactive supernanostructures for ultrasensitive monitoring of morphine. ACS Sensors. 2020, 5, 8, 2644-2651

(26) Wei Chen*, Fen Cai, Qian Wu, Yuhan Wu, Bangben Yao, and Jianguo Xu*. Prediction, evaluation, confirmation, and elimination of mat. rix effects for lateral flow test strip based rapid and on-site detection of aflatoxin B1 in tea. Food Chem., 2020, 328, 127081

(27) Jianguo Xu^#, Chao Yan^#, Xinxin Wang, Bangben Yao, Jianfeng Lu, Guodong Liu*, Wei Chen*. Ingenious design of DNA concatamers and G-quadruplex wires assisted assembly of multi-branched DNA nanoarchitectures for ultrasensitive biosensing of miRNA. Anal. Chem., 2019, 91: 9747-9753

(28) Wei Chen, Chao Yan, Lin Cheng, Li Yao, Feng Xue, Jianguo Xu*_. An ultrasensitive signal-on electrochemical aptasensor for ochratoxin A determination based on DNA controlled layer-by-layer assembly of dual gold nanoparticle conjugates, Biosens. Bioelectron., 2018,117: 845-851

(29) Jianguo Xu, Zai-Sheng Wu*, Zhenmeng Wang, Jingqing Le, Tingting Zheng, Lee Jia*. Autonomous assembly of ordered metastable DNA nanoarchitecture and in situ visualizing of intracellular microRNAs, Biomaterials, 2017, 120: 57-65

(30) Jianguo Xu, Zai-Sheng Wu*, Zhenmeng Wang, Hongling Li, Jingqing Le, and Lee Jia*, Two-wheel drive-based DNA nanomachine and its sensing potential for highly sensitive analysis of cancer-related gene, Biomaterials, 2016, 100: 110-117

专著及专利

(1)     《食品安全学》，郑州大学出版社，编委，2019年12月

(2)     Jianguo Xu, Li Yao, Lin Cheng, Chao Yan, and Wei Chen*. “Development of Aptamer-Based Electrochemical Methods.” Aptamers for Analytical Applications: Affinity Acquisition and Method Design, Willey publishing, 2018: 247-271.

(3)     徐建国; 陈伟; 闫超; 秦盼柱; 姚丽；一种检测microRNA-21的电化学传感器、其制备方法及应用； 2018-12-13, 中国专利, ZL201811523457.5（授权并转让）

(4)     徐建国; 陈伟; 秦盼柱; 闫超; 张超; 王昕昕; 高岩；2019-04-11, 中国专利,一种双重DNA机器检测microRNA-21的方法，ZL201910288202.3（授权）

(5)     徐建国; 陈伟; 张鑫磊；2022-10-25，中国专利，一种功能性核酸探针及其制备方法与应用，CN202211312408.3

(6)     徐建国；眭珠琦；郭隆华；董海燕；彭育勃；杨海东；2024-03-15，中国专利，一种检测末端脱氧核糖核酸转移酶的单链探针及应用，CN118064588A.

(7)     徐建国；郭隆华；王旗；彭育勃；眭珠琦；杨海东；2024-03-12，中国专利，一种金黄色葡萄球菌的检测方法，CN202410283073.X

(8)     徐建国；郭隆华；彭育勃；眭珠琦；杨海东；一种基于DNAzyme和滚环扩增联用的铅离子检测方法及应用，CN202410224808.1.